Quantitative analysis of gene expression systems

short Communication: Optimization of semi-quantitative RT PCR analysis for CPT I gene expression in Rainbow trout (Oncorhynchus mykiss)

short Communication: Optimization of semi-quantitative RT PCR analysis for CPT I gene expression in Rainbow trout (Oncorhynchus mykiss)

Cloning and Expression Analysis of ZmERD3 Gene From Zea mays

Background: Stresses (such as drought, salt, viruses, and others) seriously affect plant productivity. To cope with these threats, plants express a large number of genes, including several members of ERD (early responsive to dehydration) genes to synthesize and assemble adaptive molecules. But, the function of ERD3 gene hasn’t been known so far.Objectives:</strong...

Clade-Specific Quantitative Analysis of Photosynthetic Gene Expression in Prochlorococcus

Newly designed primers targeting rbcL (CO2 fixation), psbA (photosystem II) and rnpB (reference) genes were used in qRT-PCR assays to assess the photosynthetic capability of natural communities of Prochlorococcus, the most abundant photosynthetic organism on Earth and a major contributor to primary production in oligotrophic oceans. After optimizing sample collection methodology, we analyzed a ...

SAGE and the quantitative analysis of gene expression in parasites.

The nature of an organism is defined by the genes that it expresses. Genome- and expressed-sequence-tag (EST) sequencing projects are underway for many of the major parasites of humans and animals. These provide essential datasets that delineate the genes present in an organism and, in the case of ESTs, some quantitative information on gene expression. The temporal and quantitative analysis of ...